Tatjana E Kisina

52-30 Industrialny prospect, Saint-Petersburg, Russia, 195279
Tel: +7(812)234-16-69 (work)
Tel: +7(812)393-86-55 (home)
E-mail: tkissina@yandex.ru

BIRTHPLACE AND DATE Saint Petersburg, November 10, 1978

EDUCATION
2002-2005: PhD student, 3rd year, Saint Petersburg State University, Russia.
2000-2002: M.D., Saint Petersburg State University, Russia.
Major: immunology
1996-2000: B.S., Saint Petersburg State University, Russia.
Major: biology

PROFESSIONAL EXPERIENCE
2001-2005:
Junior Research scientist of the Department of Immunology in the Experimental medicine institute, RAMS, Russia. Junior Research scientist of the Laboratory of TB Immunology in the Scientific Research Institute of Phtisiopulmonology, Ministry of Health, Russia. Wrote M.D. thesis on the granulocyte activities in TB patients and a possibility of their defects correction with immunomodulator Likopid. Now studying flow cytometer methods, making PhD theses.
1999-2001:
Laboratory assistant, clinical immunology laboratory, National center of emergency and radiation medicine of EMERGCOM of Russia. Studied immunology, trained in diagnostic methods, wrote B.S. degree work on the correlation between TNF levels and neutrophils functional activity.
1996-2000:
Student, Saint-Petersburg State University, Biology and Soil Sciences Faculty, Department of Cytology and Histology. In parallel with studying at the University trained in histological tissue preparations, molecular biology methods (PCR, DNA and protein electrophoresis), has written a term work on the TCR and Ig diversity formation mechanisms.

ACTIVITIES
Skills
Laboratory techniques: cell fractioning and cell culture, ELISA, flow cytometer, PMN and lymphocyte functional analysis, molecular biology methods, circulating immune complexes assay.
Computer skills: advanced PC and Mac user, Windows, MS Office, Internet-user.
Foreign languages: English – fluent.

Publications:

1. Irina S. Freidlin, Ela A. Starickova, Evgueni I. Amtchislavski, Dmitri I. Sokolov, Tatyana E. Kisina, Sergei A. Selkov. Cytokine Control of Interaction of Endothelial cells EA.hy926 with Monocytic Cells U-937 in an In Vitro Coculture System // Clinical and Investigative Medicine. 2004. Vol. 27. N.4. P. 53B

2. Kisina Ò.Å., Freidlin I.S., Knoring B.Å., Archakova L.I., Basek Ò.S., Elkin À.V., Skvortsova L.À. Characteristics of lymphocyte phenotype in TB patients according to their proliferative response to PPD // Medical Immunology.- 2004.- Vol.6, ¹3-5. - P.312-313

3. Kisina Ò.Å. Phenotyping of circulating lymphocytes in patients with different forms of pulmonary tuberculosis. // Medical Academic Journal – 2004.- Vol.4, ¹3. – P.45

4. Kisina Ò.Å. Peripheral blood lymphocyte subsets in patients with pulmonary tuberculosis // Abstracts of international conference in Odessa State Medical Univercity “Scientists of tomorrow”. – 2004. - P.35

5. Kisina Ò.Å., Freidlin I.S., Knoring B.Å., Basek T. S., Elkin À.V., Andronova Ò.Ì. The in vitro influence of immunomodulator likopid on the peripheral blood granulocytes of tuberculosis patients // Medical Academic Journal – 2004. – Vol.3, ¹4.- P. 29-38

6. Kisina T. E., Knoring B. E., Elkin A. V., Basek T. S., Archakova L. I. Dynamic changes of PMN functional activity in tuberculosis patients. 6th John Humphrey advanced summer programme in immunology: abstracts. - 2002.- p.56-57

7. Kisina T. E., Knoring B. E., Elkin A. V., Basek T. S., Archakova L. I. Changes of neutrophil functional activity in tuberculosis patients during the treatment. // Medical Immunology – 2002. – Vol.4, ¹2. – P.239.

8. Kisina Ò.Å., Knoring B.Å., Kalinina N.Ì., Archakova L.I., Basek T. S., Neutrophil functional activity in patients with pulmonary tuberculosis // Medical Immunology – 2001. – Vol.3, ¹2. – P.221.

9. Kisina Ò.Å., Bachtin M.Yu., Syssoev Ê.À., Kalinina N.Ì. Study of TNF-mediated relations between immune system and neutrophilic granulocytes // Russian Journal of HIV/AIDS and related problems – 2000. – Vol.4, ¹1. – P.109.

(March 2006)

Brief description of current work of Tatjana Kisina.

My scientific interests lay in the field of innate immunity and immune regulation under physiologic conditions and in disease, in particular during tuberculosis. Containment and cure of tuberculosis requires an effective cell-mediated immune response, and the absence, during active tuberculosis infection, of delayed type hypersensitivity responses to M. tuberculosis antigens, defined as anergy, is associated with poor clinical outcome. It’s known that proliferative response to M.tuberculosis antigens is decreased in some tuberculosis patients. Recently regulatory suppressor cells were found in such persons. We have shown that, failure in lymphocyte functional response to tuberculin is accompanied by increased counts of CD3+CD16+CD56+ (NKT) and CD3+CD8+ cells. Maintenance of functional response to PPD is combined with increased CD3-HLA-DR+ numbers.
At present time I study an in vitro influence of IFNg and M. tuberculosis PPD (tuberculin) on NBT-test induction in PBMCs from healthy persons and PPD-normal and PPD-anergic TB patients. To date, we showed a decrease of NBT-reducing capacity in response to IFNg and PPD in most TB patients. The study comprises estimation of cytokine levels (preliminary IL-10 and IFNg) and phenotyping of blood monocytes for CD14 and CD16. Together with that I participate in collaboration project “Genetic study of normal and susceptible TB patients” of Max Planck Institute of Infection Biology (Berlin) and Institute of Phtisiopulmonology (Saint-Petersburg).