Mikhail   Mandelshtam  Ph.D., D.Sc.

DATE OF BIRTH: April, 6, 1965

POSITION: Head of the Laboratory
(Laboratory of Biochemical Genetics)

MAILING ADDRESS:
Department of Molecular Genetics
Institute of Experimental Medicine
Pavlov street, 12
197376 St.-Petersburg
RUSSIA

Tel: +7 (812) 2345606
Fax: +7 (812) 2349489

EDUCATION:
1982-1987: Leningrad (St. Petersburg) State University, Department of Biochemistry
1987-1993: Institute of Experimental Medicine, Department of Molecular Genetics, Ph.D. student (part-time)
1993: Ph.D. - Biochemistry. Ph.D. thesis entitled: "Molecular genetic study of familial hypercholesterolemia"
2006: D.Sc.: Biochemistry. D.Sc. thesis entitled: "Molecular-genetic analysis of monogenic forms of atherosclerosis and breast cancer in St.Petersburg": fulltext of abstract (729KB automand.doc)
WORK:
1987-present: Department of Molecular Genetics, Institute of Experimental Medicine Russian Academy of Medicine Sciences, St.-Petersburg, Russia
1987-1991 - Senior laboratory assistant
1991-1993 - Junior research scientist
1993-1999 - Research scientist
1999-2002 -Senior research scientist
2002 - Head of the laboratory of biochemical genetics 

FIELD OF EXPERTISE: 
Molecular genetics of familial hypercholesterolemia, familial breast cancer and glaucoma

LABORATORY SKILLS:
DNA analysis (PCR, hybridization, sequencing etc.)
DNA cloning
Protein expression

LANGUAGE SKILLS:
English, French (limited)

AWARDS:
1999 - Award and medal of European Academy of Sciences for cycle of papers on molecular genetic basis of familial hypercholesterolemia in Russia

ADDITIONAL ACTIVITY:
Taxonomy of bark-beetles (Coleoptera: Scolytidae) 

NUMBER OF PUBLICATIONS: More than 60

LIST OF THE MOST IMPORTANT PAPERS:

• Mandelshtam M.Ju., Lipovetskyi B.M., Schwartzman A.L., Gaitskhoki V.S. A novel deletion in the low density lipoprotein receptor gene in a patient with familial hypercholesterolemia from Petersburg// Human Mutation. - 1993. - Vol.2, N 4. - P. 256- 260.

• Mandel'shtam M.Yu., Lipovetskii B.M., Schvartsman A.L., Gaitskhoki V.S. Molecular heterogeneity of familial hypercholesterolemia in the St. Petersburg population// Russian Journal of Genetics. - 1995. - Vol. 31, N 4. - P. 447-452 (Translated from Genetika. - 1995. - Vol.31, N 4. - P. 521 - 527).

• Runova O.L., Mandelshtam M.Ju., Golubkov V.I., Sukonina V.E., Denisenko A.D., Gaitskhoki V.S. Expression of cDNA fragment encoding ligand-binding domain of human low density lipoprotein receptor in Escherichia coli cells//Biochemistry (Moscow). - 1997. - Vol.62, N 8. - P. 890-897 (Translated from Biokhimiya. - 1997. - Vol. 62, N 8. - P. 1037 -1045).

• Mandelshtam M.Ju., Golubkov V.I., Schur Yu.A., Lipovetsky B.M., Gaitskhoki V.S. A novel mutation 347delGCC in the human low density lipoprotein receptor gene// Russian Journal of Bioorganic Chemistry. - 1998. - Vol. 24, N 10. - P. 710-711 (Translated from Bioorganicheskaya Khimiya - 1998. - Vol. 24, N 10. - P. 798-800).

• Chakir Kh., Mandelshtam M.Ju., Shevtsov S.P., Golubkov V.I., Skobeleva N.A., Shur Y.A., Zakharova F.M., Lipovetskyi B.M., Konstantinov V.O., Denisenko A.D., Gaitskhoki V.S., Schwartz E.I. Two novel low-density lipoprotein receptor gene mutations (E397X and 347delGCC) in St.Petersburg familial hypercholesterolemia// Mol. Genet. Metabol. - 1998. - Vol.65. - P. 311-314.

• Mandelshtam M., Chakir Kh., Shevtsov S., Golubkov V., Skobeleva N., Lipovetsky B., Konstantinov V., Denisenko A., Gaitskhoki V., Schwartz E. Prevalence of Lithuanian mutation among St.Petersburg Jews with familial hypercholesterolemia// Human Mutation. - 1998. - Vol. 12. - P. 255-258.

• Durst R., Colombo R., Shpitzen S., Avi L.B., Friedlander Y., Wexler R., Raal F.J., Marais D.A., Defesche J.C., Mandelshtam M.Y., Kotze M.J., Leitersdorf E., Meiner V. Recent origin and spread of a common Lithuanian mutation, G197del LDLR, causing familial hyperholesterolemia: positive selection is not always necessary to account for disease incidence among Ashkenazi Jews// Am. J. Hum. Genet. - 2001. - Vol.68. - P. 1172-1188. Free fulltext 1172.pdf (188Kb)

• Tatishcheva Yu.A., Mandelshtam M.Yu., Golubkov V.I., Lipovetsky B.M., Gaitskhoki V.S. Four new mutations and two polymorphic variants of the low-density lipoprotein receptor gene in familial hypercholesterolemia patients from St. Petersburg// Russian Journal of Genetics. - 2001. - Vol. 37, N 9. - P. 1082-1086 (Translated from Genetika - 2001. - Vol.37, N 9. - P.1290-1295). Free English fulltext 135 KB

• Zakharova F.M., Golubkov V.I., Mandelshtam M.Ju., Lipovetskii B.M., Gaitskhoki V.S. Identification of novel missense mutation G571E, novel silent mutation H229H, nonsense mutation C74X, and four single nucleotide polymorphisms in the low-density lipoprotein receptor gene in patients with familial hypercholesterolemia from St.Petersburg// Russian Journal of Bioorganic Chemistry. - 2001. - Vol. 27, N 5. - P. 349-351 (Translated from Bioorganicheskaya Khimiya - 2001. - Vol. 27, N 5. - P. 393-396).

• Mandelshtam M. Ju., Golubkov V.I., Lamber E.P., Shapiro I.M., Brezhneva T.V., Semiglasov V.F., Lipovetsky B.M., Hanson K.P., Gaitskhoki V.S. Search for frequent mutations in genes predisposing to breast cancer// Russian Journal of Genetics. - 2001. - Vol.37, N 12. - P. 1415-1420 (Translated from Genetika - 2001. - Vol.37, N 12. - P. 1681-1686). Free English fulltext 168 KB

• Grudinina N.A., Golubkov V.I., Tikhomirova O.S., Brezhneva T.V., Hanson K.P., Vasilyev V.B., Mandelshtam M.Yu. Prevalence of widespread BRCA1 gene mutations in patients with familial breast cancer from St. Petersburg// Russian Journal of Genetics. – 2005. – Vol. 41, No 3. – P. 318 – 322 (Translated from Genetika. – 2005. – Vol. 41, N 3. – P. 405 – 410) Free English fulltext 195 KB

• Zakharova F.M., Golubkov V.I., Lipovetsky B.M., Konstantinov V.O., Mandelshtam M.Yu., Vassilyev V.B. Diagnostics of familial hypercholesterolemia in children from families with compromised inheritance // Current Pediatrics. – 2005. – Vol. 4, No 1. – P. 15 – 18 (in Russian, with English summary, P. 15).

• Zakharova F.M., Damgaard D., Mandelshtam M.Y., Golubkov V.I., Nissen P.H., Nilson G.G., Stenderup A., Lipovetsky B.M., Konstantinov V.O., Denisenko A.D., Vasilyev V.B., Faergeman O. Familial hypercholesterolemia in St.Petersburg: the known and novel mutations found in the low density lipoprotein receptor gene in Russia //BMC Med. Genet. – 2005. – Vol. 6, No. 1. – P. 6 Fulltext PDF 656KB

• Rakhmanov V.V., Nikitina N.Ya., Zakharova F.M., Astakhov Yu.S., Kvasova M.D., Vasilyev V.B., Golubkov V.I., Mandelshtam M.Yu. Mutations and polymorphisms in the genes for myocilin and optineurin as the risk factors of primary open-angle glaucoma// Russian Journal of Genetics. – 2005. – Vol. 41, No 11. – P. 1295 – 1301 (Translated from Genetika. – 2005. – Vol. 41, N 11. – P. 1567 – 1574) Free English fulltext 200 KB